Type: HPLC Application #: 108280
Detection: UV, 215nm Conditions: eluent A: 0.1% TFA in H2O, eluent B: 0.08% trifluoroacetic acid in CH3CN, gradient from 30 to 50% B in 14min, then from 50 to 65% B in 6min flow rate 1ml/min Authors: LC department Source: Macherey-Nagel
Details
Legend: Separation of pancreatic secretion of piglet, 1. trypsin + trypsinogen, 2. proelastase, 3. lipase + a-chymotrypsin, 4. chymotrypsinogen, 5. a-amylase, 6. and 7. procarboxypeptidase, sample volume 2µl.